Waveform analysis in NeuroExplorer

NeuroExplorer version 5.005 released on November 15, 2014, introduces a new analysis – Waveform Comparison. This analysis was added at a request of one of the NeuroExplorer customers who needed to compare average waveforms from one interval to another. This is a standard requirement when one does optogenetical identification of neurons, i.e. spikes are triggered by light-induced activation. To make sure this manipulation does not dramatically change the waveform of the spike, one needs to compare shape of light-triggered spikes to spontaneously occurring spikes. When you run Waveform Comparison analysis, you can display average waveforms and waveform standard deviations (shown as a gray background around averages):

Waveforms1

 

You can also display waveforms in Principal Component space:

WaveformsPC

To compare  light-triggered spikes to spontaneously occurring spikes, you need to specify two interval filters – one for stimulation periods and one for the periods with no stimulation. You can compare waveforms side-by-side:

WaveformCompValues

In the graph above the left column shows waveforms that were recorded during stimulation, the right column shows waveform averages that were recorded in periods with no stimulation.

You can also overlay averages for each neuron:

WaveformsComp

 

as well as overlay PC projections:

WfPCOverlaid

and run MANOVA to evaluate differences in the waveform projections in principal component space:

WfManova